New Grocott Stain without Using Chromic Acid

New Grocott Stain without Using Chromic Acid

We established a new "ecological" Grocott stain for demonstrating fungi, based upon a 4R principle of refusal, reduction, reuse, and recycle of waste management. Conventional Grocott stain employs environmentally harsh 5% chromic acid for oxidization. Initially, we succeeded in reducing the concentration of chromic acid from 5% to 1% by incubating the solution at 60°C and using five-fold dilute...

Conductivity and density of chromic acid solutions

The conductivity and density of chromic acid solutions were determined (<*r concentrations up to 10 molar (1,000 g/1 of Cr03 ). The density is practically a linear function of concentration. The conductivity increases to a maximum at about 5 molar and then decreases. CONTENTS Page

Chromic Acid Oxidation of Lactic Acid Catalysed by Manganese(II)

B ak o re and N a r a in 1 studied the kinetics of the oxidation of a-hydroxy acids by chromium(VI) and found that addition of Mn(II) decreases the rate of oxidation to one half of that in absence of Mn(II), when the hydrogen ion concentration is in the range 0.1 to 0.4 M. They attributed this to the disproportionation of Cr(IV) by Mn(II) ions. K em p and W a t e r s 2 observed Mn(II) catalysis...

Detection of Cryptosporidium parvum Using the Kinyoun Acid-Fast Stain

As infection with Cryptosporidium parvum becomes increasingly recognized as a cause of outbreaks of foal diarrhea, a rapid method of oocyst detection in equine feces that can be done easily in the practice setting is desirable. Protozoal identification techniques that have been described include the evaluation of stained fecal smears or preparations of floated feces, using both light and phase-...

Modified Leishman's Stain without Methyl Alcohol